Abstract
In this study, lymphoblastoid cells derived from a 79-year old individual with a history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, KLF4, LIN28, L-MYC and a p53 shRNA. The individual was homozygous for the APOE4 allele. The resulting iPS cells had a normal karyotype, retained the APOE4/4 genotype, expressed pluripotency markers, were free of genomically integrated plasmids, and could be differentiated into cell type representatives from the three germ layers in vitro.
Copyright © 2016 Michael Boutros, German Cancer Research Center, Heidelberg, Germany. Published by Elsevier B.V. All rights reserved.
MeSH terms
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Aged
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Apolipoprotein E4 / genetics*
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B-Lymphocytes / cytology
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Cell Differentiation
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Cell Line
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Cellular Reprogramming
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Dementia / genetics
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Dementia / pathology*
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Embryoid Bodies / cytology
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Embryoid Bodies / metabolism
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Genotype
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Homozygote
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Humans
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Induced Pluripotent Stem Cells / cytology*
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Induced Pluripotent Stem Cells / metabolism
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Karyotype
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Kruppel-Like Factor 4
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Microscopy, Fluorescence
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Plasmids / genetics
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Plasmids / metabolism
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Polymorphism, Single Nucleotide
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RNA Interference
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RNA, Small Interfering / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Tumor Suppressor Protein p53 / antagonists & inhibitors
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism
Substances
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Apolipoprotein E4
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KLF4 protein, human
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Kruppel-Like Factor 4
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RNA, Small Interfering
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TP53 protein, human
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Transcription Factors
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Tumor Suppressor Protein p53