Cryopreservation is widely used to generate large stocks or cell banks that can be
stored for short and long periods ready to be recovered in a functionally potent state
and used as required in a cell therapy application. Animal based reagents are usually
employed in cryopreservation media as a source of nutrients and other perceived benefits
during the post-thaw period. Current evidence suggest that the animal serum proteins
used in cryopreservation media are difficult to remove during post-thaw washing, and
any residual proteins in cells may trigger adverse reactions in the patients who receive
cell infusions or transplants. Therefore, demand is increasing for cryopreservation
media to be devoid of any animal derived components, especially when cells are intended
for cell therapy application. Human platelet lysate (HPL) provides an animal component
free option without the risks and regulatory challenges associated with animal derived
components. In this study we investigated HPL for its ability to recover functionally
potent cells following thawing and subsequent culture in cryopreservation of human
derived mesenchymal stem cells (MSC). The results indicated HPL can improve cell recovery
and functionality following thawing and provides an opportunity to develop animal
protein free regulatory compliant cryopreservation media for cell therapy applications.
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© 2015 Published by Elsevier Inc.
