Abstract
In an attempt to bring pluripotent stem cell biology closer to reaching its full potential, many groups have focused on improving reprogramming protocols over the past several years. The episomal modified Sendai virus-based vector has emerged as one of the most practical ones. Here we describe reprogramming of mesenchymal stromal/stem cells (MSC) derived from umbilical cord Wharton’s Jelly into induced pluripotent stem cells (iPSC) using genome non-integrating Sendai virus-based vectors. The detailed protocols of iPSC colony cryopreservation (vitrification) and adaption to feeder-free culture conditions are also included.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Loh YH, Yang L, Yang JC et al (2011) Genomic approaches to deconstruct pluripotency. Annu Rev Genomics Hum Genet 12:165–185
Inoue H, Yamanaka S (2011) The use of induced pluripotent stem cells in drug development. Clin Pharmacol Ther 89:655–661
Egawa N, Kitaoka S, Tsukita K et al (2012) Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104
Hou Z, Zhang J, Schwartz MP et al (2013) A human pluripotent stem cell platform for assessing developmental neural toxicity screening. Stem Cell Res Ther 4(Suppl 1):S12
Paşca SP, Portmann T, Voineagu I et al (2011) Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome. Nat Med 17:1657–1662
Miller JD, Ganat YM, Kishinevsky S et al (2013) Human iPSC-based modeling of late-onset disease via progerin-induced aging. Cell Stem Cell 13:691–705
Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Narsinh KH, Plews J, Wu JC (2011) Comparison of human induced pluripotent and embryonic stem cells: fraternal or identical twins? Mol Ther 19:635–638
Malik N, Rao M (2013) A review of the methods for human iPSC derivation. Methods Mol Biol 997:23–33
Fusaki N, Ban H, Nishiyama A (2009) Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci 85:348–362
Nishishita N, Shikamura M, Takenaka C et al (2012) Generation of virus-free induced pluripotent stem cell clones on a synthetic matrix via a single cell subcloning in the naïve state. PLoS One 7(6):e38389
Ono M, Hamada Y, Horiuchi Y et al (2012) Generation of induced pluripotent stem cells from human nasal epithelial cells using a Sendai virus vector. PLoS One 7:e42855
Kudva YC, Ohmine S, Greder LV et al (2012) Transgene-free disease-specific induced pluripotent stem cells from patients with type 1 and type 2 diabetes. Stem Cells Transl Med 1:451–461
Jin ZB, Okamoto S, Xiang P et al (2012) Integration-free induced pluripotent stem cells derived from retinitis pigmentosa patient for disease modeling
Merling RK, Sweeney CL, Choi U et al (2013) Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34+ cells. Blood 121:e98–e107
Wakao H, Yoshikiyo K, Koshimizu U et al (2013) Expansion of functional human mucosal-associated invariant T cells via reprogramming to pluripotency and redifferentiation. Cell Stem Cell 12:546–558
Kim DW, Staples M, Shinozuka K et al (2013) Wharton’s Jelly-derived mesenchymal stem cells: phenotypic characterization and optimizing their therapeutic potential for clinical applications. Int J Mol Sci 14:11692–11712
Marcus AJ, Woodbury D (2008) Fetal stem cells from extra-embryonic tissues: do not discard. J Cell Mol Med 12(3):730–742
Pappa KI, Anagnou NP (2009) Novel sources of fetal stem cells: where do they fit on the developmental continuum? Regen Med 4:423–433
Badraiq H, Devito L, Ilic D (2014) Isolation and expansion of mesenchymal stromal/stem cells from umbilical cord under chemically defined conditions. Methods Mol Biol [Epub ahead of print]
Devito L, Badraiq H, Galleu A et al (2014) Wharton’s Jelly MSC derived under chemically defined animal product-free low oxygen conditions are rich in MSCA-1+ subpopulation. Regen Med (in press)
Reubinoff BE, Pera MF, Vajta G et al (2001) Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Hum Reprod 16:2187–2194
Ilic D, Stephenson E, Wood V et al (2012) Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions. Cytotherapy 14:122–128
Stephenson E, Jacquet L, Miere C et al (2012) Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment. Nat Protoc 7:1366–1381
Acknowledgments
The study was supported by the studentship to C.M. from the Medical Research Council, UK.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Miere, C., Devito, L., Ilic, D. (2014). Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells. In: Turksen, K., Nagy, A. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 1357. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_163
Download citation
DOI: https://doi.org/10.1007/7651_2014_163
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3054-8
Online ISBN: 978-1-4939-3055-5
eBook Packages: Springer Protocols