Culturing and Cryobanking Human Neural Stem Cells

Jeremy M Crook; Eva Tomaskovic-Crook

doi:10.1007/978-1-4939-6921-0_15

Culturing and Cryobanking Human Neural Stem Cells

Methods Mol Biol. 2017:1590:199-206. doi: 10.1007/978-1-4939-6921-0_15.

Authors

Jeremy M Crook^{1

2

3}, Eva Tomaskovic-Crook^{4

5}

Affiliations

¹ ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.
² Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia. jcrook@uow.edu.au.
³ Department of Surgery, St. Vincent's Hospital, The University of Melbourne, Fitzroy, Victoria, 3065, Australia. jcrook@uow.edu.au.
⁴ ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia.
⁵ Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, 2522, Australia.

PMID: 28353272
DOI: 10.1007/978-1-4939-6921-0_15

Abstract

The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

Keywords: Cryopreservation; Culture; Expansion; Freezing; Human neural stem cells; Passaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Specimen Banks
Cell Culture Techniques / methods
Cell Differentiation / physiology
Cells, Cultured
Cryopreservation / methods
Humans
Neural Stem Cells / cytology*
Neurogenesis / physiology
Neuroglia / cytology
Neurons / cytology